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Akt/p27kip1 Pathway Is Not Involved in Human Insulinoma Tumorigenesis
Escrito por Dr. Oscar Domingo BrunoAgradecemos la gentileza del Dr. Oscar. D. Bruno por el envío de este valioso material.
Insulinomas are pancreatic neuroendocrine tumors (pNET), usually benign. Akt/p27kip1 is an intracellular pathway overexpressed in many pNET. There are no data regarding its expression in human insulinomas. We aimed to investigate the expression of Akt and p27kip1 in 24 human insulinomas and to compare them to their expression in normal surrounding islets. Staining was performed on embedded paraffin tissue using polyclonal antibodies against total Akt, p-Akt, p27kip1, and pp27kip1. p-Akt was the predominant form in insulinomas; they presented lower Akt and p-Akt expression than normal islets in 83.3% and 87.5% of tumors, respectively. p27kip1 and pp27kip1 were mainly cytoplasmic in both insulinomas and normal tissue. Cytoplasmic pp27kip1 staining was higher in insulinomas and surprisingly nearly half of the insulinomas also presented nuclear p27kip1 (p=0029). No differences were observed in the subcellular localization of p27kip1 and activation of Akt between benign and malignant insulinomas. The low expression of Akt seen in insulinomas might explain the usual benign behavior of this type of pNET. Cytoplasmic p27kip1 in both insulinomas and normal islet cells could reflect the low rate of replication of beta cells, while nuclear p27kip1 would seem to indicate stabilization and nuclear anchoring of the cyclin D-Cdk4 complex. Our data seem to suggest that the Akt pathway is not involved in human insulinoma tumorigenesis.
Última actualización el 29 de Octubre de 2019
Mas envíos del Dr. Oscar Bruno

Agradecemos la gentileza del Dr. Oscar. D. Bruno por el aporte de este valioso material y nos congratulamos por su generoso compromiso para futuros envíos, muchas gracias Maestro. Existe la idea generalizada de que la acromegalia es una enfermedad benigna e infrecuente. Sin embargo, el paciente acromegálico ve comprometida su vida a causa de complicaciones cardiovasculares y metabólicas. Por otra parte, trabajos recientes muestran que su frecuencia parece mucho mayor que lo supuesto previamente. Dado que los signos y síntomas de la enfermedad se instalan lenta e insidiosamente, existe una demora considerable en su diagnóstico. Habitualmente, los pacientes han consultado diversos...
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Agradecemos la gentileza del Dr. Oscar. D. Bruno por el envío de este valioso material. The main reason that led me to write this paper was the presentation of a poster at the 12 International Thyroid Conference in Kyoto, Japan (2000), about “Complications of Thyroidectomy in Patients with Radiation-Induced Thyroid Neoplasms”. This poster was illustrated with a picture of the well-known opera singer Amelita Galli-Curci. At that time, I became aware of what was called among American neck surgeons “the nerve of Galli-Curci”. But, what was the story behind it?
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Agradecemos la gentileza del Dr. Oscar. D. Bruno por el envío de este valioso material. The role of mechanical forces influencing the growth of a pituitary adenoma is poorly understood. In this paper we report the case of a young man with hyperprolactinaemia and an empty sella secondary to hydrocephalia, who developed a macroprolactinoma following the relief of high intraventricular pressure. Learning points: {jb_bluebox}• The volume of a pituitary tumour may be influenced not only by molecular but also by local mechanical factors. • Intratumoural pressure, resistance of the sellar diaphragm and intracranial liquid pressure may play a role in the final size of...
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Read more
Agradecemos la gentileza del Dr. Oscar. D. Bruno por el envío de este valioso material. Evidence-based treatment guidelines have undoubtedly advanced medical practice and supported optimal management of acromegaly, but their application may be hampered by limited access to the latest treatment options.
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